Composite
Part:BBa_K4891005:Design
Designed by: Kexin Fei Group: iGEM23_Guangzhou-MedX (2023-06-27)
aroGfbr-aroB-aroD-aroE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2175
Illegal XhoI site found at 934 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1965
Illegal AgeI site found at 2509 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2362
Illegal SapI.rc site found at 241
Design Notes
For one thing, the usage of AroGfbr could alleviate the feedback inhibition of aromatic amino acids. For another thing, AroB optimization enable to enhance expression level. As a result, shikimate biosynthesis is improved owing to the enhanced metabolic flow.
Source
There are four genes for converting PEP and E4P into shikimate, including aroG (encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase), aroB (encoding 3-dehydroquinate synthase), aroD (encoding 3-dehydroquinate dehydratase), and aroE (encoding shikimate dehydrogenase), and these genes originate from Escherichia coli.