Composite

Part:BBa_K4891005:Design

Designed by: Kexin Fei   Group: iGEM23_Guangzhou-MedX   (2023-06-27)


aroGfbr-aroB-aroD-aroE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2175
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1965
    Illegal AgeI site found at 2509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2362
    Illegal SapI.rc site found at 241


Design Notes

For one thing, the usage of AroGfbr could alleviate the feedback inhibition of aromatic amino acids. For another thing, AroB optimization enable to enhance expression level. As a result, shikimate biosynthesis is improved owing to the enhanced metabolic flow.


Source

There are four genes for converting PEP and E4P into shikimate, including aroG (encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase), aroB (encoding 3-dehydroquinate synthase), aroD (encoding 3-dehydroquinate dehydratase), and aroE (encoding shikimate dehydrogenase), and these genes originate from Escherichia coli.

References